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dapi nuclear staining  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology dapi nuclear staining
    Dapi Nuclear Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+staining+with+dapi/DAPI/pm42869916-314-14-20
    Average 96 stars, based on 1459 article reviews
    dapi nuclear staining - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: PDE4 inhibition by difamilast regulates filaggrin and loricrin expression via keratinocyte proline-rich protein in human keratinocytes.
    Article Snippet: The slides were then washed with PBS before incubation with anti-rabbit secondary antibody (Alexa Fluor 546; Molecular Probes, Eugene, OR, USA) for 1 h at room temperature. .. After nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI), the slides were mounted with UltraCruz mounting medium (Santa Cruz Biotechnology). .. All samples were analyzed using a Leica TCS SP8 confocal laser scanning microscope (Leica, Wetzlar, Germany).

    Article Title: MiR-4270 acts as a tumor suppressor by directly targeting Bcl-xL in human osteosarcoma cells
    Article Snippet: DNA content after staining of trypsinized cells was performed by nuclear staining with propidium iodide (Sigma-Aldrich, Saint-Louis, MO, USA) using a Cytoflex S Flow Cytometer (Beckman Coulter France SAS, Paris, France) as previously described ( ). .. Morphological characterization of detached cells and trypsinized cells by nuclear staining with DAPI (4’,6-diamidino-2-phenylindole, dilactate) (Santa Cruz Biotechnology, Dallas, TX, USA) was carried out as previously described ( ). .. Protein levels of both detached and adherent cells were analyzed by immunoblotting with PARP (9542), Bcl-xL (2764) and Bak (3814) antibodies from Cell Signaling Technology (Ozyme, Saint-Quentin-en-Yvelines, France), with Mcl-1 (S-19), GAPDH (FL-335) and β-Tubulin (D-10) antibodies from Santa Cruz Biotechnology (Dallas, TX, USA), and with the Bcl-2 (M0887) antibody from DAKO (Agilent, Santa Clara, CA, USA) as previously described ( , ).

    Article Title: Eucalyptol Ameliorates Retinal Microvascular Defects through Modulating ER Stress and Angiopoietin–Tie Signaling in Diabetic Eyes
    Article Snippet: .. Nuclear staining was performed with 4′,6-diamidino-2-phenylindole (DAPI, Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Each slide was mounted in VectaMount mounting medium (Vector Laboratories, Burlingame, CA, USA).

    Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis.
    Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with DAPI nuclear staining (1:1000, Cat# sc-3598, Santa Cruz). .. The following antibodies were used in human tissues: anti-CD33 (Polyclonal, 1:100, Cat# bs-2042R, Bioss), Nature Communications | (2026) 17:10746 12 anti-HLA-DR (LN3, 1:100, Cat# LS-B8114, LSBio), anti-CD20 (H1, 1:500, Cat# 555677, BD Biosciences), anti-CD56 (123A8, 1:1000, Cat# NBP244713, Novus), anti-CD11c (Polyclonal, 1:300, Cat# orb13554, Biorbyt), anti-CD3 (PS1, 1:50, Cat# ab699, Abcam), anti-CD14 (4B4F12, 1:100, Cat# ab182032, Abcam), anti-CD16 (2Q1240, 1:50, Cat# sc-70548, Santa Cruz), anti-CD123 (6H6, 1:100, Cat# 306002, BioLegend), anti-CD274/ PD-L1 (E1L3N, 1:100, Cat# 85164SF, Cell Signaling), anti-CD200 (Polyclonal, 1:100, Cat#AF2724, R&DSystems), anti-Podoplanin (Polyclonal, 1:100, Cat# AF3670, R&D Systems).

    Article Title: Aesculetin Suppresses Airway Epithelial Barrier Disruption Induced by Inhalation of Urban Coarse Particulate: Involvement of Neutrophil Elastase and Protease-Activated Receptor‑2
    Article Snippet: .. Nuclear staining was performed with 4′,6-diamidino-2-phenylindole (DAPI, Santa Cruz Biotechnology). .. Each slide was mounted in VectaMount mounting medium (Vector Laboratories, Burlingame, CA, USA).

    Article Title: System and method for combination of cold atmospheric plasma treatment with radiation and chemotherapy
    Article Snippet: Some of the tissues procured from the patient were immediately cryosectioned using Leica cryostat. .. 5-6 μm sections at −20° C. were stained with H&E and double immunofluorescence for localization of colon stem cell marker human CD44 FITC (Bio Legend), anti-TRAIL receptorl (Santa Cruz) and second antibody anti-alexaflour 594 or 488 (molecular probes), respectively was used along with nuclear counterstaining with DAPI (Vectashield, Molecular Probes). .. Appropriate isotype controls (Life Technologies) were maintained.

    Article Title: Monoamine Oxidase Contributes to Valvular Oxidative Stress: A Prospective Observational Pilot Study in Patients with Severe Mitral Regurgitation.
    Article Snippet: The tissue expression of MAO was quantified in frozen sections of human mitral valves using MAO-A (Abcam, Cambridge, UK, ab126751) and MAO-B (Abcam, Cambridge, UK, ab175136) primary antibodies and Alexa Fluor labeled secondary goat anti-rabbit antibody (Invitrogen, Waltham, MA, USA, A32731), respectively, as previously described [24]. .. Nuclear staining was obtained with DAPI (Santa Cruz, Dallas, TX, USA, SC3598). .. The slides were examined using an Olympus Fluoview FV1000 confocal microscope (Hachioji, Tokyo, Japan).

    Article Title: Umbelliferone preserves skin epidermal barrier integrity in diabetes-induced keratinocytes by regulating actin dynamics and suppressing ROS-mediated apoptosis.
    Article Snippet: .. Nuclear staining was performed with 4′,6-diamidino-2-phenylindole (DAPI, Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Each slide was mounted with VectaMount mounting medium (Vector Laboratories, Burlingame, CA, USA).

    Incubation:

    Article Title: CD200 + fibroblasts prime the immunoregulatory function of granulocytic myeloid-derived suppressor cells in inflammatory arthritis.
    Article Snippet: .. Secondary antibodies were stained for 1 h at room temperature followed by incubation with DAPI nuclear staining (1:1000, Cat# sc-3598, Santa Cruz). .. The following antibodies were used in human tissues: anti-CD33 (Polyclonal, 1:100, Cat# bs-2042R, Bioss), Nature Communications | (2026) 17:10746 12 anti-HLA-DR (LN3, 1:100, Cat# LS-B8114, LSBio), anti-CD20 (H1, 1:500, Cat# 555677, BD Biosciences), anti-CD56 (123A8, 1:1000, Cat# NBP244713, Novus), anti-CD11c (Polyclonal, 1:300, Cat# orb13554, Biorbyt), anti-CD3 (PS1, 1:50, Cat# ab699, Abcam), anti-CD14 (4B4F12, 1:100, Cat# ab182032, Abcam), anti-CD16 (2Q1240, 1:50, Cat# sc-70548, Santa Cruz), anti-CD123 (6H6, 1:100, Cat# 306002, BioLegend), anti-CD274/ PD-L1 (E1L3N, 1:100, Cat# 85164SF, Cell Signaling), anti-CD200 (Polyclonal, 1:100, Cat#AF2724, R&DSystems), anti-Podoplanin (Polyclonal, 1:100, Cat# AF3670, R&D Systems).

    Immunofluorescence:

    Article Title: System and method for combination of cold atmospheric plasma treatment with radiation and chemotherapy
    Article Snippet: Some of the tissues procured from the patient were immediately cryosectioned using Leica cryostat. .. 5-6 μm sections at −20° C. were stained with H&E and double immunofluorescence for localization of colon stem cell marker human CD44 FITC (Bio Legend), anti-TRAIL receptorl (Santa Cruz) and second antibody anti-alexaflour 594 or 488 (molecular probes), respectively was used along with nuclear counterstaining with DAPI (Vectashield, Molecular Probes). .. Appropriate isotype controls (Life Technologies) were maintained.

    Marker:

    Article Title: System and method for combination of cold atmospheric plasma treatment with radiation and chemotherapy
    Article Snippet: Some of the tissues procured from the patient were immediately cryosectioned using Leica cryostat. .. 5-6 μm sections at −20° C. were stained with H&E and double immunofluorescence for localization of colon stem cell marker human CD44 FITC (Bio Legend), anti-TRAIL receptorl (Santa Cruz) and second antibody anti-alexaflour 594 or 488 (molecular probes), respectively was used along with nuclear counterstaining with DAPI (Vectashield, Molecular Probes). .. Appropriate isotype controls (Life Technologies) were maintained.



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